ASTM E1259 - 10

Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C

This standard is a part of ASTM Book of Standards Volume 11.05

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Annotation and contents

Source of this annotation and contents: ASTM International.

Significance and Use

Guide D6469 details the types of problems associated with uncontrolled microbial growth in fuels and fuel systems. Treatment with effective antimicrobial agents is one element of contamination control strategy.

The procedure should be used to evaluate the relative efficacy of microbicides in liquid fuels boiling below 390°C. The effect of environmental conditions, such as a variety of fuel additives, metal surfaces, and climatology, are variables that can be included in specific tests using this protocol.

This practice addresses product performance issues only. Regulatory Agencies restrict and control the use of both pesticides (in the U.S.: 40 CFR 152) and fuel additives (40 CFR 79). Regardless of performance in this method, antimicrobials must only be used in compliance with applicable regulations. Specific industries, for example, the aviation industry, may place further restrictions on chemicals used for fuel treatment.

1. Scope

1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751, and D7467), system deterioration, or both.

1.2 Knowledge of microbiological techniques is required for these procedures.

1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

D396 Specification for Fuel Oils

D910 Specification for Aviation Gasolines

D975 Specification for Diesel Fuel Oils

D1655 Specification for Aviation Turbine Fuels

D2069 Specification for Marine Fuels

D2880 Specification for Gas Turbine Fuel Oils

D3699 Specification for Kerosine

D4814 Specification for Automotive Spark-Ignition Engine Fuel

D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component

D6293 Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic(O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography

D6469 Guide for Microbial Contamination in Fuels and Fuel Systems

D6729 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography

D6733 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography

D6751 Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels

D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels--Filtration and Culture Procedures

D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water

D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

D7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)

E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria

NACE Standard

TM0172 Determining Corrosive Properties of Cargoes in Petroleum Product Pipelines Item No. 21204, available from NACE International (NACE), 1440 South Creek Dr., Houston, TX 77084-4906, http://www.nace.org.

Federal Standards

40CFRPart152 Pesticide Registration and Classification Procedures

Alternate designation

  • ASTM E 1259
  • ASTM E1259-10
  • ASTM E 1259-10